FOXO3 Is Expressed in Ovarian Tissues and Acts as an Apoptosis Initiator in Granulosa Cells of Chickens

Figure 3

FOXO3 knockdown in chicken granulosa cells. (a) The relative mRNA expression level of FOXO3 after the knockdown. (b) Western blotting analysis of FOXO3 protein levels after FOXO3 knockdown. (c) The relative mRNA expression levels of the apoptosis-related genes after FOXO3 knockdown. (d) Western blot analysis of cleaved PARP-1 and caspase 3 in control and FOXO3 knockdown cells. β-Actin served as a reference. Data are expressed as means ± SEM (n = 3 independent cell cultures). P<0.05; P<0.01.

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