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Figure 2: The effect of ES and BAPTA-AM on cell proliferation and migration. (a)EdU incorporation was carried on immediately for 2h after ES. Analysis of (b) G1/S/G2 distributions of cell cycle, (c) cell migration, and (d) the expression of proliferation and cell cycle related proteins 24h later after ES treatment. P<0.001; NS: no significance compared with the control group. Every experiment was repeated for 3 times. Control: control group; ES: electrical stimulation group. BAPTA-AM: BAPTA-AM treatment group. B+ES: BAPTA-AM and electrical stimulation treatment group.