Figure 4: The effect of ES and Ca2+ on AKT/GSK3β signaling activation. The protein expression of AKT, GSK3β, p-AKT, and p-GSK3β was detected after ES and BAPTA-AM treatment. <0.05 and <0.001 compared with control. Every experiment was repeated for 3 times. Control: control group; ES: electrical stimulation group. BAPTA-AM: BAPTA-AM treatment group. B+ES: BAPTA-AM and electrical stimulation treatment group.