Research Article

Hepatoprotective Effect of Jianpi Huoxue Formula on Nonalcoholic Fatty Liver Disease Induced by Methionine-Choline-Deficient Diet in Rat

Figure 4

JPHX inhibits hepatic fibrosis in MCD diet-fed rats, as suggested by profibrotic gene expression level, Sirius red staining, and α-SMA Expressions level. (a) Collagen I and MMP-9 mRNA expression levels were detected by qRT-PCR. The relative amounts of mRNA were normalized to β-actin. (b) Sirius red staining showed noticeable pericellular fibrosis in the MCD group. Representative liver sections from different groups. Original magnification × 200. (c) Paraffin-embedded sections were immunostained with an α-SMA antibody. Representative liver sections from different groups. Original magnification × 400. Values are shown as mean ± SD. MCS n=6, MCD n=9, and JPHX treatment groups n=7. #p<0.05, ##p<0.01, and ###p<0.001, MCD diet vehicle group versus MCS diet group. p<0.05, p<0.01, and p<0.001, MCD diet vehicle group versus JPHX treatment groups.

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