A High Throughput HPLC-MS/MS Method for Antihypertensive Drugs Determination in Plasma and Its Application on Pharmacokinetic Interaction Study with Shuxuetong Injection in Rats

Table 3

Precision and accuracy of the analytes in rat plasma (n = 6).

Compounds	Spiked; concentration (ng/mL)	Intraday			Interday
		Measured (ng/mL)	RE; (%)	RSD (%)	Measured (ng/mL)	RE (%)	RSD (%)
metoprolol tartrate	20	21.16 ± 0.91	5.8	4.3	21.16 ± 0.64	5.8	3.0
	400	431.98 ± 10.65	8.0	2.5	427.53 ± 12.12	6.9	2.8
	4000	3725.53 ± 113.06	-6.9	3.0	3784.38 ± 153.35	-5.4	4.1
hydrochlorothiazide	25	24.07 ± 1.70	-3.7	7.1	26.07 ± 2.37	4.3	9.1
	500	531.80 ± 28.55	6.4	5.4	524.21 ± 29.44	4.8	5.6
	5000	4551.15 ± 42.31	-9.0	0.9	4510.25 ± 147.90	-9.8	3.3
	5	5.27 ± 0.33	5.5	6.2	5.34 ± 0.30	6.9	5.6
nifedipine	100	100.64 ± 2.47	0.6	2.5	106.67 ± 5.20	6.7	4.9
	1000	909.20 ± 13.18	-9.1	1.5	902.93 ± 43.30	-9.7	4.8
	50	56.37 ± 3.12	12.7	5.5	55.30 ± 1.94	10.6	3.5
valsartan	1000	1074.78 ± 24.16	7.5	2.3	1083.06 ± 23.84	8.3	2.2
	10000	8647.62 ± 294.77	-13.5	3.4	8813.82 ± 376.14	-11.9	4.3