Effects of Pyrene on Human Liver HepG2 Cells: Cytotoxicity, Oxidative Stress, and Transcriptomic Changes in Xenobiotic Metabolizing Enzymes and Inflammatory Markers with Protection Trial Using Lycopene

Table 1

Primer sequences of the target genes used in this study.

Target	Primer sequence
CYP1A1	F-5′-CTATCTGGGCTGTGGGCAA-3′
	R-5′-CTGGCTCAAGCACAACTTGG-3′
CYP1A2	F-5′-CATCCC CCACAGCACAACAA-3′
	R-5′-TCCCACTTGGCCAGGACTTC-3′
CYP1B1	F-5′-CTTTCGGCCACTACTCGGAG-3′
	R-5′-CTCGAGGACTTGGCGGCT-3′
UGT1A6	F-5′-CATGATTGTTATTGGCCTGTAC-3′
	R-5′-TCTGTGAAAAGAGCATCAAACT-3′
GSTA1	F-5′-CAGCAAGTGCCAATGGTTGA-3′
	R-5′-TATTTGCTGGCAATGTAGTTGAGAA-3′
NQO1	F-5′-GGATTGGACCGAGCTGGAA-3′
	R-5′-AATTGCAGTGAAGATGAAGGCAAC-3′
MDR1	F-5′-GGGAAGAGCACAACAGTCCA-3′
	R-5′-ATGTGACTGCTGATCACCGC-3′
MRP2	F-5′-AGAGAGCTGCAGAAAGCCAG-3′
	R-5′-CATCTTCCAGGACAAGGGCA-3′
HO1	F-5′-ATGGCCTCCCTGTACCACATC-3′
	R-5′-TGTTGCGCTCAATCTCCTCCT-3′
GSTO1	F-5′-AGGACGCGTCTAGTCCTGAA-3′
	R-5′-TTCCCTGGGTATGCTTCATC-3′
SOD1	F-5′-GCAGGTCCTCACTTTAATCCTCT-3′
	R-5′-ATCGGCCACACCATCTTTGT-3′
CAT	F-5′-TGAAGATGCGGCGAGACTTT-3′
	R-5′-TGGATGTAAAAAGTCCAGGAGGG-3′
TNFα	F-5′-GAAGAGTTCCCCAGGGACCT-3′
	R-5′-GGGTTTGCTACAACATGGGC-3′
COX2	F-5′-GAGGGCCAGCTTTCACCAA-3′
	R-5′-TGTGGGAGGATACATCTCTCCA-3′
AhR	F-5ʹ-ATCACCTACGCCAGTCGCAAG-3ʹ
	R-5ʹ-AGGCTAGCCAAACGGTCCAAC-3ʹ
Nrf2	F-5′-CTTGGCCTCAGTGATTCTGAAGTG-3′
	R-5′-CCTGAGATGGTGACAAGGGTTCTA-3′
GAPDH	F-5′-TCCAAAATCAAGTGGGGCGA-3′
	R-5′-TGATGACCCTTTTGGCTCCC-3′