Stable Reference Gene Selection for RT-qPCR Analysis in Synechococcus elongatus PCC 7942 under Abiotic Stresses
The relative expression level of the cyanobacterial core clock gene kaiC under different experimental conditions was normalized to the most stable paired reference genes, the most stable single reference gene, and the most unstable reference gene. Templates were collected for 0–24 h under each treatment condition. (a) NTC. (b) NaCl. (c) HO. (d) HL. Bars represent the standard error from three biological replicates. The white bars under the X-axis indicate , the subjective daytime, and the gray bars under the X-axis indicate , the subjective night time.
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