Research Article
Stable Reference Gene Selection for RT-qPCR Analysis in Synechococcus elongatus PCC 7942 under Abiotic Stresses
Table 4
Expression stability ranking of the nine candidate reference genes.
| Method | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 |
| (a) Ranking order under ALL sample (better–good–average) | geNorm | prs/secA | | rnpA | 16S | ilvD | ppc | petB | rimM | rnpB | NormFinder | prs | secA | ilvD | rnpA | 16S | ppc | rimM | petB | rnpB | BestKeeper | secA | prs | 16S | petB | rnpA | ilvD | ppc | rimM | rnpB | Comprehensive ranking | prs | secA | 16S | rnpA | ilvD | petB | ppc | rimM | rnpB | (b) Ranking order under NTC condition (better–good–average) | geNorm | prs/secA | | ppc | ilvD | 16S | rnpA | petB | rnpB | rimM | NormFinder | secA | ppc | rnpA | prs | 16S | ilvD | petB | rnpB | rimM | BestKeeper | 16S | secA | rnpA | ppc | prs | ilvD | petB | rnpB | rimM | Comprehensive ranking | secA | ppc | prs | 16S | rnpA | ilvD | petB | rnpB | rimM | (c) Ranking order under NaCl stress (better–good–average) | geNorm | ppc/prs | | ilvD | rimM | rnpA | petB | secA | 16S | rnpB | NormFinder | rimM | ilvD | rnpA | petB | prs | ppc | secA | 16S | rnpB | BestKeeper | petB | rnpA | rimM | 16S | prs | ilvD | ppc | secA | rnpB | Comprehensive ranking | rimM | rnpA | ilvD | petB | prs | ppc | 16S | secA | rnpB | (d) Ranking order under HO stress (better–good–average) | geNorm | ppc/prs | | ilvD | rnpA | secA | petB | rnpB | rimM | 16S | NormFinder | rnpA | ilvD | ppc | prs | secA | petB | rnpB | rimM | 16S | BestKeeper | rnpA | 16S | secA | ilvD | ppc | petB | prs | rnpB | rimM | Comprehensive ranking | rnpA | ilvD | ppc | prs | secA | petB | 16S | rnpB | rimM | (e) Ranking order under HL stress (better–good–average) | geNorm | prs/secA | | petB | ppc | rnpA | ilvD | rimM | 16S | rnpB | NormFinder | rnpA | prs | secA | rimM | ppc | ilvD | petB | 16S | rnpB | BestKeeper | petB | prs | rnpA | secA | rimM | ppc | ilvD | 16S | rnpB | Comprehensive ranking | prs | secA | petB | rnpA | ppc | rimM | ilvD | 16S | rnpB |
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