Glutathione Peroxidase 1 Promotes NSCLC Resistance to Cisplatin via ROS-Induced Activation of PI3K/AKT Pathway

Figure 6

BAY 11-7082, a NF-κB inhibitor, downregulated intracellular ROS and reduced cisplatin resistance. (a) A549 and H1975 NSCLC cells treated with BAY 11-7082 showed downregulation of GPX1 expression, and correspondingly AKT phosphorylation was inhibited. The effects of BAY 11-7082 were similar to the effects observed in the same cell lines treated with GPX1 siRNA. ((b), (c), and (d)) In H1975 cells, suppression of GPX1 expression by both siGPX1 and BAY 11-7082 enhanced intracellular ROS accumulation and subsequently restored the sensitivity of the treated cells to cisplatin. ((e), (f), and (g)) A549 cells showed the same findings as H1975 cells when the cells were treated with siGPX1 and BAY 11-7082.

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