TY - JOUR A2 - Palomares, Oscar AU - Xian, Zhemin AU - Jin, Guangyu AU - Li, Hongmei AU - Jiang, Jingzhi AU - Wang, Chongyang AU - Zhu, Lianhua AU - Jin, Zhehu AU - Li, Liangchang AU - Piao, Hongmei AU - Zheng, Mingyu AU - Yan, Guanghai PY - 2019 DA - 2019/01/20 TI - Imperatorin Suppresses Anaphylactic Reaction and IgE-Mediated Allergic Responses by Inhibiting Multiple Steps of FceRI Signaling in Mast Cells: IMP Alleviates Allergic Responses in PCA SP - 7823761 VL - 2019 AB - This study is to investigate the effects of imperatorin (IMP) on allergic responses mediated by mast cells, both in vitro and in vivo. Passive cutaneous anaphylaxis (PCA) model was established. Histological detection was performed to assess the ear histology. ELISA and Western blot analysis were used to detect the levels of corresponding cytokines and signalling pathway proteins. IMP decreased the leakage of Evans blue and the ear thickness in the PCA models, in a dose-dependent manner, and alleviated the degranulation of mast cells. Moreover, IMP reduced the expression of TNF-α, IL-4, IL-1β, IL-8, and IL-13. Furthermore, IMP inhibited the phosphorylation levels of Syk, Lyn, PLC-γ1, and Gab2, as well as the downstream MAPK, PI3K/AKT, and NF-κB signaling pathways. In addition, IMP inhibited the mast cell-mediated allergic responses through the Nrf2/HO-1 pathway. IMP attenuates the allergic responses through inhibiting the degranulation and decreasing the expression levels of proinflammatory cytokines in the mast cells, involving the PI3K/Akt, MAPK, NF-κB, and Nrf2/HO-1 pathways. SN - 2314-6133 UR - https://doi.org/10.1155/2019/7823761 DO - 10.1155/2019/7823761 JF - BioMed Research International PB - Hindawi KW - ER -