TY - JOUR
A2 - Palomares, Oscar
AU - Xian, Zhemin
AU - Jin, Guangyu
AU - Li, Hongmei
AU - Jiang, Jingzhi
AU - Wang, Chongyang
AU - Zhu, Lianhua
AU - Jin, Zhehu
AU - Li, Liangchang
AU - Piao, Hongmei
AU - Zheng, Mingyu
AU - Yan, Guanghai
PY - 2019
DA - 2019/01/20
TI - Imperatorin Suppresses Anaphylactic Reaction and IgE-Mediated Allergic Responses by Inhibiting Multiple Steps of FceRI Signaling in Mast Cells: IMP Alleviates Allergic Responses in PCA
SP - 7823761
VL - 2019
AB - This study is to investigate the effects of imperatorin (IMP) on allergic responses mediated by mast cells, both in vitro and in vivo. Passive cutaneous anaphylaxis (PCA) model was established. Histological detection was performed to assess the ear histology. ELISA and Western blot analysis were used to detect the levels of corresponding cytokines and signalling pathway proteins. IMP decreased the leakage of Evans blue and the ear thickness in the PCA models, in a dose-dependent manner, and alleviated the degranulation of mast cells. Moreover, IMP reduced the expression of TNF-α, IL-4, IL-1β, IL-8, and IL-13. Furthermore, IMP inhibited the phosphorylation levels of Syk, Lyn, PLC-γ1, and Gab2, as well as the downstream MAPK, PI3K/AKT, and NF-κB signaling pathways. In addition, IMP inhibited the mast cell-mediated allergic responses through the Nrf2/HO-1 pathway. IMP attenuates the allergic responses through inhibiting the degranulation and decreasing the expression levels of proinflammatory cytokines in the mast cells, involving the PI3K/Akt, MAPK, NF-κB, and Nrf2/HO-1 pathways.
SN - 2314-6133
UR - https://doi.org/10.1155/2019/7823761
DO - 10.1155/2019/7823761
JF - BioMed Research International
PB - Hindawi
KW -
ER -