BioMed Research International / 2019 / Article / Fig 2

Research Article

Imperatorin Suppresses Anaphylactic Reaction and IgE-Mediated Allergic Responses by Inhibiting Multiple Steps of FceRI Signaling in Mast Cells: IMP Alleviates Allergic Responses in PCA

Figure 2

IMP reduced degranulation, histamine release, and intracellular calcium level of mast cells. (a) RBL-2H3 cells and purified RPMCs were treated with indicated concentrations of IMP. Cell viability was measured by MTT assay. (b) Effects of IMP on the degranulation of RPMCs (1000× magnification). (A) Normal RPMCs in HEPES-Tyrode buffer; (B) RPMCs sensitized with 10 μg/ml anti-DNP IgE for 6 h and challenged with 100 ng/ml DNP-HAS; (C) RPMCs incubated with 40 μM IMP at 37°C for 30 min; (D) Prior to the challenge with 100 ng/ml DNP-HAS, preincubated with 40 μM IMP at 37°C for 30min. (c) RBL-2H3 cells were stimulated with 10 μg/ml anti-DNP IgE for 6 h and challenged with 100 ng/ml DNP-HAS. The histamine release of RBL-2H3 cells was measured by the radioenzymatic method. (d) RPMCs were stimulated with 10 μg/ml anti-DNP IgE for 6 h and challenged with 100 ng/ml DNP-HAS. Calcium uptake was measured by the radioenzymatic method. (e) RPMCs were stimulated with 10 g/ml anti-DNP IgE for 6 h and challenged with 100 ng/ml DNP-HAS. The histamine release of RPMCs was measured by the radioenzymatic method. (f) Results of quantitative analysis of mast cell degranulation. Compared with the Con group, P<0.05, ∗∗P<0.01; compared with the IgE+Ag group, #P<0.05, ##P<0.01.
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