Imperatorin Suppresses Anaphylactic Reaction and IgE-Mediated Allergic Responses by Inhibiting Multiple Steps of FceRI Signaling in Mast Cells: IMP Alleviates Allergic Responses in PCA

Figure 4

Effects of IMP on FcεRI-mediated signaling pathway in RBL-2H3 cells. RBL-2H3 cells were divided into the Con, IgE+Ag, and IgE+Ag+IMP groups. RBL-2H3 cells were stimulated with 10 μg/ml anti-DNP IgE for 6 h and challenged with 100 ng/ml DNP-HAS, in the absence or presence of IMP (10, 20, and 40 μM, respectively). Levels of Syk, Lyn, Gab2, and PLC-γ1were detected by Western blot analysis. Compared with the Con group, ^∗P<0.05, ^∗∗P<0.01; compared with the IgE+Ag group, ^#P<0.05, ^##P<0.01. (b) Illustration on the possible relationship between IMP and FcεRI-mediated signaling pathway. IMP disabled the phosphorylation of Syk, Lyn, Gab2, and PLCg1. We demonstrated that IMP suppressed the allergic inflammation mediated by mast cells, and we suppose that this effect may be mediated by the Fc epsilon RI-dependent signaling pathway.

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