BioMed Research International / 2019 / Article / Tab 1 / Research Article
Phylogenetic Analysis and Screening of Antimicrobial and Antiproliferative Activities of Culturable Bacteria Associated with the Ascidian Styela clava from the Yellow Sea, China Table 1 Compositions of the media for the isolation of bacteria associated with ascidian Styela clava .
Media Composition 2216E Peptone 5 g; yeast extract 1 g; FePO4 0.01 g; agar 18 g; natural seawater 1 L; pH 7.6. M1 Soluble starch 10 g; yeast extract 4 g; peptone 2 g; agar 18 g; natural seawater 750 mL; deionized water 250 mL. M2 Glycerol 6 mL; arginine 1 g; K2 HPO4 ·3H2 O 1 g; MgSO4 ·7H2 O 0.5 g; agar 18 g; natural seawater 1 L. M3 L-asparagine 0.1 g; casein peptone 2 g; K2 HPO4 ·3H2 O 0.05 g; MgSO4 ·7H2 O 0.1 g; FeSO4 ·7H2 O 0.01 g; agar 18 g; nature seawater 1 L; pH 7.0. M4 Yeast extract 5 g; L- asparagine 1 g; glycerol 10 mL; K2 HPO4 ·3H2 O 1 g; KNO3 5 g; agar 18 g; nature seawater 1 L; pH 7.5-8.5. M5 Agar 18 g; natural seawater 1 L. M8 Aspartic acid 0.1 g; casein peptone 2 g; sodium propionate 4 g; K2 HPO4 ·3H2 O 0.05 g; MgSO4 ·7H2 O 0.1 g; FeSO4 ·7H2 O 0.01 g; agar 18 g; natural seawater 1 L; pH 7.0. M12 Soluble starch 10 g; casein 0.5 g; K2 HPO4 ·3H2 O 0.5 g; NaCl 20 g; deionized water 1 L; agar 18 g; pH 7.0. M13 Yeast extract 4 g; soluble starch 15 g; K2 HPO4 ·3H2 O 1 g; MgSO4 ·7H2 O 0.5 g; agar 18 g; natural seawater 1 L; pH 7.0. M18 Glucose 10 g; asparagine 1 g; K2 HPO4 ·3H2 O 1 g; FeSO4 ·7H2 O 0.001 g; ZnSO4 ·7H2 O 0.01 g; agar 18 g; natural seawater 750 mL; deionized water 250 mL; pH 7.0. M19 Glycerol 10 g; acid hydrolyzed casein 0.3 g; KNO3 2 g; NaC1 2 g; K2 HPO4 ·3H2 O 2 g; MgSO4 ·7H2 O 0.05 g; FeSO4 ·7H2 O 0.01 g; CaCO3 0.2 g; agar 18 g; natural seawater 750 mL; deionized water 250 mL; pH 7.0. Peptone–beef extract medium Beef extract 5.0 g; peptone 10.0 g; NaCl 5.0 g; agar 18 g; nature seawater 1 L; pH 7.4-7.6.
