Research Article

Wnt Signaling Protects against Paclitaxel-Induced Spiral Ganglion Neuron Damage in the Mouse Cochlea In Vitro

Figure 2

Paclitaxel treatment induced caspase-mediated apoptosis in SGNs. (a) The diagram of the assay for (b)–(e). The middle turn cochleae and SGN explants from P3 C57BL/6 WT mice were cultured and incubated with 30 μM paclitaxel for 48 h and then used for immunostaining. (b) Representative confocal images of SGNs labeled with DAPI (grey), Tuj1 (green), and TUNEL (red). (c) Statistical data showed that TUNEL/Tuj1 double-positive cells were significantly increased in the paclitaxel-treated group. (d) Representative confocal images of cleaved-caspase 3 (red) and Tuj1 (green) immunofluorescence. (e) Statistical data showed that the cleaved-caspase 3/Tuj1 double-positive SGNs in the paclitaxel-treated group were statistically increased compared to the control group. vs. control group.