Laboratory and Clinical Evaluation of DNA Microarray for the Detection of Carbapenemase Genes in Gram-Negative Bacteria from Hospitalized Patients

Figure 1

(a) The microarray layout of the target carbapenemase genes that are detected in this study. (b) Principle of hybridization and CL imaging of microarray. (A) Captured probes were fixed to the aldehyde-chip surface, (B) denatured PCR products were hybridized with capture probes, (C) horseradish peroxidase modified streptavidin was bond to biotin incorporated in hybridization, and (D) adding HRP substrates luminal and H₂O₂ and (E) CL signal was detected by catalyzed substrates.

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