Research Article

Development and Clinical Evaluation of Loop-Mediated Isothermal Amplification (LAMP) Assay for the Diagnosis of Human Visceral Leishmaniasis in Brazil

Figure 2

Analytical sensitivity of loop-mediated isothermal amplification (LAMP) for the detection of Leishmania infantum DNA. Turbidity increases in the positive reaction due to the generation of magnesium pyrophosphate. Tube 1, 1ng; Tube 2, 100pg; Tube 3, 10pg; Tube 4, 1pg; Tube 5, 100fg; Tube 6, 10fg; Tube 7, 1fg; Tube 8, 0.1fg.