Development and Clinical Evaluation of Loop-Mediated Isothermal Amplification (LAMP) Assay for the Diagnosis of Human Visceral Leishmaniasis in Brazil

Figure 3

Specificity of the K26-LAMP assay for detection of Leishmania donovani complex DNA. Visual detection of the turbidity of the reactions solutions under natural light (top row); LAMP products studied by polyacrylamide gel electrophoresis (bottom row). Lane M, 100 bp DNA ladder; lane and tube 1, L. donovani; lane and tube 2, L. infantum; lane and tube 3, L. amazonensis; lane and tube 4, L. braziliensis; lane and tube 5, L. guyanensis; lane and tube 6, Schistosoma mansoni; lane and tube 7, Trypanosoma cruzi; lane and tube 8, Toxoplasma gondii.