Phenothiazinium Dyes Are Active against Trypanosoma cruzi In Vitro

Figure 2

Inhibitory activity of phenothiazinium dyes combinations against T. cruzi. Amastigotes were incubated with seven dilutions (2 × IC₅₀, 1.6 × IC₅₀, 1.3 × IC₅₀, 0.7 × IC₅₀, 0.5 × IC₅₀, and 0.2 × IC₅₀) of MB + BZ (a), NMB + BZ (b), TBO + BZ (c), DMMB + BZ (d), MB + NMB (e), MB + TBO (f), MB + DMMB (g), NMB + TBO (h), NMB + DMMB (i), TBO + DMMB (j), and the proliferation measured after CPRG assay. As references, isolated compounds of each combination were evaluated concomitantly in the same plate. The inhibition percentage was calculated in comparison to the nontreated group and the IC₅₀ and CI values were achieved using Compusyn software.

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