BioMed Research International / 2019 / Article / Fig 4

Research Article

PEGylated Polyethylenimine Derivative-Mediated Local Delivery of the shSmad3 Inhibits Intimal Thickening after Vascular Injury

Figure 4

Gene silencing effect of PEG-Et 1:1 and inhibition effect of PEG-Et 1:1/shSmad3 polyplex on VSMCs proliferation. (a) qRT-PCR analysis of Smad3 mRNA expression, GAPDH served as internal standard. n = 3. (b) Semiquantitative densitometric analysis of relative Smad3 protein level. n = 3. (c) Western blot analysis of Smad3 protein expression, GAPDH served as internal standard. (d) Effects of PEG-Et 1:1-mediated delivery of shSmad3 and adSmad3 on VSMCs proliferation in vitro. The cell number was expressed as a percentage of the absorbance to that of the control group (PEG-Et 1:1/scrambled shRNA) measured by CCK-8. Control: PEG-Et 1:1/scrambled shRNA. n = 6. Each bar shows as mean ± SD. P < 0.05; P < 0.01; P < 0.001.

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