Integrated Analysis of mRNA Expression, CpG Island Methylation, and Polymorphisms in the MITF Gene in Ducks (Anas platyrhynchos)

Table 1

Primer pairs used for PCR amplification.

Primer name	Primer sequence (5′-3′)	Product size (bp)	Tm (°C)
PCR-HaeII-RFLP	F: TATGAGTGCCTGCTGCTACCT	485	59.5
	R: GTTGATGGCAGGTGTCTGG
PCR-HaeIII-RFLP	F: TTGTACGGGCACCGATAT	457	52.6
	R: CTTGAGGAGCAGCGATTA
PCR-ApoI-RFLP	F: CCTACAGAGTCAGAAGCAAGAG	329	57.2
	R: TTCCAAGAAGTCCAGATAAACAG
PCR-HinfI-RFLP	F: GCCACGTCAACAGTCCCACA	540	56.5
	R: GGCGGTCACTCACAGCATCAG
CpG island normal primer	F: TTCCCGGCATCTGAACAAAG	983	60.5
	R: TCTCAACAGCAAAGGCACCC
CpG island 1 BSP primer	F: GGTTGTTTTTAAAGTGTAATTTTGT	256	58.5
	R: AAACTCCCCTTAATATTTCATCCTC
CpG island 2 BSP primer	F: AGAGGATGAAATATTAAGGGGAGTT	222	60.5
	R: ACCTACCTACCAAAAAACTCATTTC
TYR	F: TTACATGGTCCCCTTTATTC	182	60
	R: CAATCACAGCTGCACCAACC
MITF	F: CCCAGTTCATGCAGCAGAGAGT	268	60
	R: CCAGGCGGCATGACATGATCAC
β-Actin	F: AACTGGGATGACATGGAGAAGA	189	60
	R: ATGGCTGGGGTGTTGAAGGT

Note. RFLP, restriction fragment length polymorphism; BSP, bisulfite sequencing PCR; Tm, melting temperature.