Research Article

Integrated Analysis of mRNA Expression, CpG Island Methylation, and Polymorphisms in the MITF Gene in Ducks (Anas platyrhynchos)

Table 3

Genotype distribution and genetic polymorphisms of the duck MITF gene.

SNP positionBreedGenotype frequency (number of birds)Allele frequencyPIC
CCCTTTCT

g.312C>TPTB0.35 (39)0.54 (61)0.11 (12)0.6210.3790.360
PTW0.99 (106)0.01 (1)0 (0)0.9950.0050.009
LC0.87 (93)0.13 (14)0 (0)0.9350.0650.115
LS0.44 (47)0.46 (49)0.09 (10)0.6750.3250.343

g.32813G>AGGGAAAGA
PTB0.36 (40)0.51 (57)0.13 (15)0.6120.3880.362
PTW1.00 (107)0 (0)0 (0)1.0000.0000.000
LC0 (0)0.11 (12)0.89 (95)0.0560.9440.100
LS0.78 (83)0.21 (22)0.01 (1)0.8870.1130.181

g.39807T>GTTTGGGTG
PTB0 (0)0.03 (3)0.97 (109)0.0130.9870.026
PTW0.79 (85)0.19 (20)0.02 (2)0.8880.1120.179
LC0.02 (2)0 (0)0.98 (105)0.0190.9810.036
LS0 (0)0.01 (1)0.99 (105)0.0050.9950.009

g.40862G>AGGGAAAGA
PTB0 (0)0.02 (2)0.98 (110)0.0090.9910.018
PTW0.86 (92)0.12 (13)0.02 (2)0.9210.0790.136
LC0 (0)0 (0)1.00 (107)0.0001.0000.000
LS0 (0)0.17 (18)0.83 (88)0.0850.9150.143

Note. PTB means Putian Black duck (N = 112), PTW means Putian White duck (N = 107), LC means Liancheng White duck (N = 107), and LS means Longsheng Jade-green duck (N = 106). PIC means polymorphism information content. PIC < 0.25 indicates a low level of polymorphism, 0.25 < PIC < 0.5 indicates a medium level of polymorphism, and PIC > 0.5 indicates a high level of polymorphism.