Evaluation of the Efficacy of a Recombinant Adenovirus Expressing the Spike Protein of Porcine Epidemic Diarrhea Virus in Pigs

Figure 2

Antibody responses against PEDV vaccination from serum samples. Pigs were vaccinated at 0 dpv and then challenged orally with 2 mL of diluted virus containing 1 × 10⁵ TCID₅₀ highly virulent genotype GIIb PEDV strain CH/HNPJ/2017 at 28 dpv. Serum samples were collected at 0, 7, 14, 21, and 28 dpv. (a) PEDV-specific IgG antibody responses against PEDV from serum samples from vaccinated pigs determined using an indirect ELISA. (b) PEDV-specific IgA antibody responses against PEDV from serum samples from vaccinated pigs determined using an indirect ELISA. (c) PEDV-specific VN antibody responses elicited by vaccination with the recombinant virus rAd-PEDV-S. Neutralizing antibody titers were calculated as the reciprocal of the highest serum dilution that inhibited CPEs. Error bars represent the SEM. Statistical significance among different experimental groups was determined using one-way ANOVA with Tukey’s multiple-comparison test. The asterisk indicates a significant difference, with the value less than 0.05.

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