Optimization and Standardization of Thermal Treatment as a Plasma Prefractionation Method for Proteomic Analysis

Figure 1

Optimization of the temperature and incubation time for thermal treatment. (a) At a fixed incubation time of 20 min, various temperatures from 65°C to 95°C affected the plasma patterns, with the prominent protein band of human serum albumin (HSA) (69 kDa) being markedly decreased at 95°C. By varying the incubation time from 5 min to 30 min at the selected temperature of 95°C, the depletion level of HSA was constant at 20 min of treatment. (b) The protein band pattern of the TS soluble fraction obtained from the optimal thermal treatment at 95°C for 20 min was compared to that of untreated plasma and the thermolabile precipitated protein fraction by 12.5% SDS-PAGE (10 μg/lane).

(a)

(b)