Calcium Oscillatory Behavior and Its Possible Role during Wound Healing in Bovine Corneal Endothelial Cells in Culture

Figure 8

Effect of EGTA, ARL, and ATP on the apoptotic response during wound healing. (a) A Hoechst-stained micrograph of a wounded BCE monolayer after 8 hours of healing. The arrows indicate apoptotic bodies at the wound border revealed by intense staining due to nuclei compaction. (b) 2 h after wounding, during the first stage of apoptosis (FSA), incorporation of EGTA determined an approximately 200% increase in the apoptotic index AI (defined as the number of apoptotic bodies AB per millimeter of wound edge, AB/mm). On the contrary, ARL produced a decrease of about 50%. (c) 20 h after wounding, during the second stage of apoptosis (SSA), none of the treatments provoked a significant effect. (d) BCE monolayers were incubated with different ATP concentrations and treated with CPA and EGTA to completely abolish the FCW (see Materials and methods for details). RAI is the corresponding apoptotic index relative to control. The asterisk () indicates significant difference with respect to control (p < 0.05).

(a)

(b)

(c)

(d)