PC-3-Derived Exosomes Inhibit Osteoclast Differentiation by Downregulating miR-214 and Blocking NF-B Signaling Pathway

Figure 2

PC-3-derived exosomes inhibit osteoclast differentiation. (a) TRAP staining of osteoclasts treated with 0, 10, 30, or 50 ng exosomes/1000 cells. (b) Relative expression of miR-214 in osteoclasts treated with 0, 10, 30, or 50 ng exosomes/1000 cells. (c) qRT-PCR of CTSK, NFATc1, and ACP5 in osteoclasts after treatment with 0, 10, 30, or 50 ng exosomes/1000 cells. (d) Western blotting of CTSK, NFATc1, and ACP5 in osteoclasts after treatment with 0, 10, 30, or 50 ng exosomes/1000 cells.

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