Research Article

Usnea Acid as Multidrug Resistance (MDR) Reversing Agent against Human Chronic Myelogenous Leukemia K562/ADR Cells via an ROS Dependent Apoptosis

Figure 3

MDR Reversing Activity of UA Was Inhibited by NAC. Cells were treated with UA(4 μM), Adr UA(4 μM), and UA plus Adr (4 μM, respectively) for 48 hr before examination; medium with same concentration of DMSO was used as control. Cell viability was detected by CCK8 assay (a). Apoptosis related proteins were analyzed by western blot (b). Intracellular generation of ROS (c) and accumulation of Adr (d) were observed by confocal microscopy and flow cytometry. Columns: values are expressed as mean ± SD.
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