LncRNA-TWIST1 Promoted Osteogenic Differentiation Both in PPDLSCs and in HPDLSCs by Inhibiting TWIST1 Expression
Figure 3
LncRNA-TWIST1 inhibited the TWIST1 expression and upregulated the osteogenic differentiation of PPDLSCs. (a–c) The mRNA levels of TWIST1, β-catenin, and cyclin D in PPDLSCs infected by the lncRNA-TWIST1-overexpressing lentivirus. (d) The mRNAs expression of osteogenic genes in PPDLSCs infected by the lncRNA-TWIST1-overexpressing lentivirus. (e–g) The mRNA expression levels of TWIST1, β-catenin, and cyclin D in PPDLSCs infected by the lncRNA-TWIST1-knockdown lentivirus. (h) The mRNAs expression of osteogenic genes in PPDLSCs infected by the lncRNA-TWIST1-knockdown lentivirus. (i) Alizarin red and ALP staining for osteogenic differentiations of PPDLSCs. (j) Quantitative measurement of Alizarin red staining. (k) ALP activity assay (P< 0.05, P< 0.01). Mean ± SEM.