CRISPR/Cas9 Knockout of Bak Mediates Bax Translocation to Mitochondria in response to TNFα/CHX-induced Apoptosis

Figure 1

Overexpression of the antiapoptotic protein Bcl-2 inhibited TNFα/CHX-induced apoptosis. (a) Top panels show cytochrome c release from the mitochondria (right column) to the cytosol (left column). Second panels show Bax translocation from the cytosol (left panel) to the mitochondria (right panel). Third panels show Bak release from the mitochondria (right column) to the cytosol (left column). Fourth panels using antibody COX I to confirm the mitochondria remained intact using mitochondria isolation kit. Bottom panels show loading control β-actin. (b) After 24 h of transfection, Bcl-2 levels were significant (top panel, lanes 3 and 4). Western blots for PARP (second); caspase-8, -9, and -3 (panels 3–5); and loading control β-actin (bottom panel) are shown.