Prolyl 4-Hydroxylase Domain Protein 3-Inhibited Smooth-Muscle-Cell Dedifferentiation Improves Cardiac Perivascular Fibrosis Induced by Obstructive Sleep Apnea

Figure 4

PHD3 overexpression degrades and inactivates HIF-1α. (a) RT-qPCR analysis of HIF-1α mRNA (a1), Western blot analysis and quantification of HIF-1α (a2 a3), and PHD3 (a2 a4). (b) Representative images of immunofluorescence staining with antibodies to HIF-1α (red). Nuclei were counterstained with DAPI (blue) (original magnification ×400 bars=20 μm). (c) Western blot analysis and quantitative analysis of α-SMA (c1 c2), OPN (c1 c3). p<0.05 versus control; p<0.01 versus control; p<0.01 versus IH+LvNC; p<0.05 versus IH; p<0.05 versus IH+shNC.

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