Identification of a Ribosomal Protein RpsB as a Surface-Exposed Protein and Adhesin of Rickettsia heilongjiangensis

Figure 2

Detection of RpsB in R. heilongjiangensis using immunoelectron microscopy. Host cells that had been infected with R. heilongjiangensis were incubated with antisera against RpsB (a) and TrxA (b), and subsequently immunolabeled with colloidal gold particles (10 nm) using standard procedures. The cells were then observed by transmission electron microscopy. The average number of black spots of RpsB that were located on the inner membrane (im), on outer membrane (om), or in the cytoplasm (cp) were calculated (c).

(a)

(b)

(c)