Melittin Inducing the Apoptosis of Renal Tubule Epithelial Cells through Upregulation of Bax/Bcl-2 Expression and Activation of TNF-α Signaling Pathway

Figure 5

The expression of Bax and Bcl-2 in HK-2 cells. The mRNA expression of Bax and Bcl-2 in HK-2 cells (a). P (compared with the control): N>0.05, <0.05, <0.01. P (intergroup comparison): n>0.05, &<0.05. The protein expression of Bax and Bcl-2 in HK-2 cells detected by simple Western assays (b). A: the control group, B: the melittin group, C: the TNF-α group, D: the melittin+TNF-α group. P (compared with the control): N>0.05, <0.05, <0.01. P (intergroup comparison): n>0.05. The protein expression of Bax and Bcl-2 in HK-2 cells detected by IF stains (c). IF: immunofluorescent. Magnification×200. P (compared with the control): <0.01, <0.001.