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BioMed Research International
/
2019
/
Article
/
Tab 2
/
Research Article
Duplex TaqMan Hydrolysis Probe-Based Molecular Assay for Simultaneous Detection and Differentiation of
Burkholderia pseudomallei
and
Leptospira
spp. DNA
Table 2
List of primers and probes used in this study.
Target
Type
Sequence (5′ →3′)
Source
Leptospira
spp.
Forward primer
ACTGAGACACGGTCCATACT
[
19
]
Reverse primer
TAGTTAGCYGGTGCTTTAGGYA
Probe
FAM-ACGGGAGGCAGC-ZEN-AGTTAAGAATCTTGC-IBFQ
B. pseudomallei
Forward primer
CCTGGGAGAGCGAGATGTT
[
20
]
Reverse primer
GCTGGATGAGAAGAAAGTCC
Probe
TexRed-CCACGCACGGCGGAGATTCT-IBRQ