Follistatin-Like 1 Attenuates Ischemia/Reperfusion Injury in Cardiomyocytes via Regulation of Autophagy
Effect of autophagy after FSTL1 pretreatment on the viability and apoptosis of H9C2 cells exposed to hypoxia/ischemia conditions. (a) Cell viability was determined with the CCK-8 assay under different conditions. P < 0.05, #P < 0.05 vs. the hypoxia/ischemia group. (b) Representative immunofluorescence images of H9C2 cells exposed to different conditions. Proliferating cells were stained with EdU, and the total cells were stained with Hoechst 3344. (c) The percentage of EdU-positive cells among the total cells was calculated and analyzed. The 0 group: the cells had no treatment under hypoxic/ischemic. P < 0.05, #P < 0.05 vs. the hypoxia/ischemia group. (d) Apoptosis as determined by flow cytometry after Annexin V/PI staining. The 0 group: the cells had no treatment under hypoxic/ischemic. (e) Percentage of apoptotic cells. The 0 group: the cells had no treatment under hypoxic/ischemic. P < 0.05, #P < 0.05 vs. the hypoxia/ischemia group. All experiments were repeated at least three times.
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