BioMed Research International / 2019 / Article / Fig 4

Research Article

Follistatin-Like 1 Attenuates Ischemia/Reperfusion Injury in Cardiomyocytes via Regulation of Autophagy

Figure 4

Effect of FSTL1 treatment after rapamycin or 3-MA pretreatment on the viability and apoptosis of H9C2 cells exposed to hypoxia/ischemia conditions. (a) and (b) Cell viability was determined with the CCK-8 assay under different conditions. The 0 group: the cells had no treatment under hypoxic/ischemic. (c) Apoptotic cells as determined by flow cytometry after Annexin V/PI staining. (d) Percentage of apoptotic cells. P < 0.05, P < 0.01 vs. the hypoxia/ischemia group. (e) Apoptotic cells as determined by flow cytometry after Annexin V/PI staining. (f) Percentage of apoptotic cells. P < 0.05, P < 0.01 vs. the hypoxia/ischemia group. All experiments were repeated at least three times.

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