The Appropriate Marker for Astrocytes: Comparing the Distribution and Expression of Three Astrocytic Markers in Different Mouse Cerebral Regions
Figure 9
The expression of NDRG2, GFAP, and S100β in different cerebral regions of different aged male mice. (a)-(f). The levels of NDRG2, GFAP, and S100β protein in cortex, hippocampus, and thalamus of the male mice aged 1 month, 3 months, 6 months, 9 months, and 12 months were determined by Western blot analysis. Representative blots and quantitative analysis were shown. (g)-(i) The levels of NDRG2, GFAP and S100β mRNA in cortex, hippocampus, and thalamus of the male mice aged 1 month, 3 months, 6 months, 9 months, and 12 months were determined by qPCR analysis. The quantitative analysis was shown. The mean of 1 month was standardized to 1. Results were presented as the mean ± SD and analyzed by Student’s t-tests. n=6. p<0.05, p<0.01, and p<0.001. 1 m = 1 month; 3 m = 3 months; 6 m = 6 months; 9 m = 9 months; 12 m = 12 months.