Research Article

[Retracted] Fbxw7 and Skp2 Regulate Stem Cell Switch between Quiescence and Mitotic Division in Lung Adenocarcinoma

Figure 4

Effects of Fbxw7 and Skp2 on the cell cycle and the expression of c-myc and p27. (a) The protein expression of c-myc was determined by Western blotting assays in A549 cells after transfection with Fbxw7 siRNA. (a) The mRNA expression of c-myc was determined by qRT-PCR in A549 cells after transfection with Fbxw7 siRNA. (c) The protein expression of p27 was determined by Western blotting assays in A549 cells after transfection with Skp2 siRNA. (d) The mRNA expression of p27 was determined by qRT-PCR in A549 cells after transfection with Skp2 siRNA. (e) Effects of the Fbxw7 siRNA and Skp2 siRNA on A549 cell cycle distribution. Cell cycle distribution of G0/G1 and S phases was measured by flow cytometry. (f) The percentage of cells at different cycle phases was calculated. All the results are shown as mean ± SD (n = 3, p < 0.05, significantly different from control group).

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