Simultaneous Determination of Six Uncaria Alkaloids in Mouse Blood by UPLC–MS/MS and Its Application in Pharmacokinetics and Bioavailability

Table 4

Stability of rhynchophylline, isorhynchophylline, corynoxeine, isocorynoxeine, hirsutine, and hirsuteine in mouse blood.

Compound	Concentration (ng/mL)	Autosampler ambient		Ambient 2 h		-20°C 30 d		Freeze-thaw
		Accuracy	RSD	Accuracy	RSD	Accuracy	RSD	Accuracy	RSD
Rhynchophylline	4	98.8	6.7	102.6	7.3	87.2	11.2	81.4	11.1
	90	106.7	2.3	101.1	1.4	103.4	9.5	104.4	9.3
	900	105.4	6.0	95.0	8.2	98.3	8.6	96.2	10.7
Isorhynchophylline	4	105.6	7.1	97.5	11.4	99.7	9.2	106.4	8.0
	90	103.7	8.2	108.9	8.2	107.1	13.5	104.4	12.1
	900	101.4	4.6	93.5	6.9	89.4	4.8	105.5	9.9
Corynoxeine	4	96.8	5.5	103.9	2.4	96.1	11.3	91.5	13.9
	90	97.6	2.8	101.5	6.5	101.8	5.5	98.1	7.0
	900	101.7	4.1	105.2	5.7	107.9	2.8	105.7	9.0
Isocorynoxeine	4	103.5	6.4	106.0	8.9	102.9	7.5	107.7	9.7
	90	97.2	6.4	97.2	4.5	96.5	7.2	95.0	11.5
	900	103.1	3.7	98.7	3.9	101.5	8.9	107.7	6.6
Hirsutine	4	101.9	10.6	95.3	12.9	111.6	11.2	92.0	12.1
	90	97.2	8.2	103.9	11.7	95.9	7.7	103.2	11.0
	900	100.2	8.5	102.3	4.8	110.6	11.4	92.3	12.1
Hirsuteine	4	96.6	9.3	95.1	11.3	99.0	12.6	109.8	13.6
	90	99.6	7.8	107.5	9.0	93.0	11.4	92.0	10.2
	900	100.1	5.1	96.4	5.9	103.2	11.9	99.8	9.9