Involvement of the G-Protein-Coupled Receptor 4 in the Increased Expression of RANK/RANKL/OPG System and Neurotrophins by Nucleus Pulposus Cells under the Degenerated Intervertebral Disc-Like Acidic Microenvironment

Figure 1

The expression of GPR4 and cAMP accumulation in the degenerated IVD-like acidic condition. The expression level of GPR4 in nucleus pulposus cells was determined by RT-PCR and western blot test (a, b) in pH 7.2 and pH 6.5. cAMP accumulation in nucleus pulposus cells was measured in pH 7.2 and pH 6.5 (C). Data were represented as of 6 independent experiments performed in triplicate; Student’s -test were used to find out differences. ( VS. control in pH 7.2; VS. control in pH 7.2).

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