BioMed Research International / 2020 / Article / Fig 1

Research Article

Emodin Alleviates Hydrogen Peroxide-Induced Inflammation and Oxidative Stress via Mitochondrial Dysfunction by Inhibiting the PI3K/mTOR/GSK3β Pathway in Neuroblastoma SH-SY5Y Cells

Figure 1

Emodin alleviates apoptosis of H2O2-induced neuroblastoma cell SH-SY5Y. SH-SY5Y cells were treated with emodin of different concentrations (0, 10, 20, 50, and 100 μM) for 24 h and with H2O2 (200 μM) for 1 h. The experiment was divided into the control, 0, 10, 20, 50, and 100 μM groups. (a) The cell viability rate was determined using the MTT. (b) The apoptosis of SH-SY5Y was determined using V-FITC/PI. (c) The protein expression of caspase-3. (d) The protein expression of caspase-9. (e) LDH release was measured in the same experimental conditions. “” means compared with the control group at . GAPDH was used as an invariant internal control for calculating protein fold changes.

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