BioMed Research International / 2020 / Article / Fig 2

Research Article

Emodin Alleviates Hydrogen Peroxide-Induced Inflammation and Oxidative Stress via Mitochondrial Dysfunction by Inhibiting the PI3K/mTOR/GSK3β Pathway in Neuroblastoma SH-SY5Y Cells

Figure 2

Emodin alleviates the inflammation of H2O2-induced neuroblastoma cell SH-SY5Y. SH-SY5Y cells were treated with emodin at a concentration of 50 μM for 24 h and with H2O2 (200 μM) for 1 h. The experiment was divided into the control group, H2O2 group, and H2O2+emodin group. The levels of proinflammatory factors were detected by ELISA and Western blot. (a–c) The level of IL-6, TNF-α, and NO in the control group, H2O2 group, and H2O2+emodin group. (d) The protein expression of COX-2 and iNOS in the control group, H2O2 group, and H2O2+emodin group. “” means compared with the control group at , and “#” means compared with the H2O2 group at . GAPDH was used as an invariant internal control for calculating protein fold changes.
(a)
(b)
(c)
(d)

Article of the Year Award: Outstanding research contributions of 2020, as selected by our Chief Editors. Read the winning articles.