Research Article

Neuroepithelial Cell Transforming Gene 1 Acts as an Oncogene and Is Mediated by miR-22 in Human Non-Small-Cell Lung Cancer

Figure 3

NET1 was directly suppressed by miR-22. (a, b) TargetScan and miRDB bioinformatics analysis revealed that NET1 is predicted to be a target of miR-22. (c) Predicted miR-22 target sequences in the wild-type and mutant 3′UTR regions of NET1. (d) The Luciferase activity assay illustrated that overexpression of miR-22 could decrease the intensity of the fluorescence in both A549 and H1299 cells transfected with the NET1 3′UTR WT vector, while it was invalid with the NET1 3′UTR MUT vector (n = 6). (e, f) The expression of NET1 in both mRNA and protein in NSCLC cell lines was significantly reduced following upregulation of miR-22, which was determined by qRT-PCR and western blotting. β-Actin was used as a loading control. (n = 3). .
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