Research Article

Anti-Inflammatory and Antioxidant Effect of Eucommia ulmoides Polysaccharide in Hepatic Ischemia-Reperfusion Injury by Regulating ROS and the TLR-4-NF-κB Pathway

Figure 5

EUP attenuates the rise in ROS caused by I/R. ROS levels in liver frozen sections were detected using DHE probes. When the DHE probes were oxidized, they were combined with DNA and showed red fluorescence, and the nuclei were stained in blue with DAPI. Compared with the sham group, a large amount of red fluorescence was observed in the IR group, indicating that a large amount of ROS was produced in the liver when HIRI occurred, while a significant decrease in red substances was observed in the EUPH group. Staining results showed that pretreatment with 320 mg/kg EUP significantly reduced the rise in liver ROS caused by I/R (original magnification of ).