The Significance of Circular RNA DDX17 in Prostate Cancer

Figure 1

Upregulation of circDDX17 suppresses the migration, EMT, and proliferation of prostatic cancer cells. (a) DDX17 expression in PCa and normal tissues was harvested form TCGA database. Red box represented PCa samples and gray box represented normal tissues. . (b) The circDDX17 expression in prostate cancer tissue samples and corresponding adjacent nontumor tissue samples was determined by qPCR. (c) The circDDX17 expression in PC-3 cell lines transfected with pcDNA-NC or pcDNA-circDDX17 was determined by qPCR. (d) Relative cell proliferation in PC-3 cell lines transfected with pcDNA-NC or pcDNA-circDDX17 was detected by CCK8. (e) The protein E-cadherin and Vimentin expression in 22Rv1 and PC-3 cell lines transfected with pcDNA-NC or pcDNA-circDDX17 was measured by Western blotting. (f) The invasion of PC-3 cell lines transfected with pcDNA-NC or pcDNA-circDDX17 was determined by transwell assays. (g) Relative cell numbers of invasion in PC-3 cell lines are shown. (h) The colonizing ability of PC-3 cell lines transfected with pcDNA-NC or pcDNA-circDDX17 was determined by colony formation assays. (i) Relative cell numbers of colony formation in 22Rv1 cell lines are shown. , , and .

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