Research Article

The Heat Shock Protein 70 Plays a Protective Role in Sepsis by Maintenance of the Endothelial Permeability

Figure 1

HSP70 level was detected after treatment with LPS. (a) ELISA was used to detect the secretory HSP70 after treatment with different concentrations of LPS (0, 200, 500, 1000, and 2000 ng/ml) for 24 h. (b, c) Real-time PCR (b) and western blot (c) were used to analyze the mRNA and protein levels of HSP70 after treatment with different concentrations of LPS (0, 200, 500, 1000, and 2000 ng/ml) for 24 h. ; ; vs. 0 ng/ml LPS. (d) ELISA was used to detect the secretory HSP70 after treatment with 500 ng/ml LPS for 0, 6, 12, and 24 h. (e, f) Real-time PCR (e) and western blot (f) were used to analyze the mRNA and protein levels of HSP70 after treatment with 500 ng/ml LPS for 0, 6, 12, and 24 h. ; ; vs. 0 h.
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