Research Article

The Heat Shock Protein 70 Plays a Protective Role in Sepsis by Maintenance of the Endothelial Permeability

Figure 3

The effects induced by the HSP70 inhibitor could be blocked by the p38 inhibitor. (a) Expression levels of HSP70, p38, and p-p38 were analyzed in the cells treated with 0, 5, 10, and 20 μM Apoptozole. (b) CCK-8 assay was used to measure cell viability in HUVEC under treatments with Apoptozole and/or SB203580. (c) ELISA was used to detect the productions of IL-1β, IL-6, and TNF-α after treatments. (d, e) Endothelial permeability was measured using TEER assay and FITC-Dextrans. (f) Expression levels of E-cadherin, occludin, ZO-1, p38, and p-p38 were measured by western blot. ; vs. control; +++ vs. Apoptozole.
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