Nrp-1 Mediated Plasmatic Ago2 Binding miR-21a-3p Internalization: A Novel Mechanism for miR-21a-3p Accumulation in Renal Tubular Epithelial Cells during Sepsis

Figure 3

Nrp-1 expression and distribution of TECs in different groups. (a) Representative Western Blot results of Nrp-1 in TECs of either control or sepsis (12 h: 12 hours after CLP) rat model). (b) Quantitative analysis of RT-PCR results of Nrp-1 mRNA transcription level in TECs of either control or sepsis (12 h: 12 hours after CLP) rat model). (c) Representative fluorescent results of Nrp-1 expression and distribution of TECs treated with different plasma. (d) Quantitative analysis of fluorescent results of Nrp-1 expression. (e) Quantitative analysis of RT-PCR results of Nrp-1 mRNA transcription level in TECs treated with different plasma. (f) Representative flow cytometry results of membrane Nrp-1 on TECs treated with different plasma. (g) Quantitative analysis of flow cytometry results of membrane Nrp-1 on TECs treated with different plasma. (h) Representative Western Blot results of membrane Nrp-1 on TECs treated with different plasma ( vs. the control; Ctrl: control; NP: normal plasma; 12 h: septic plasma from rats 12 hours after CLP).

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