Trifolium Flavonoids Overcome Gefitinib Resistance of Non-Small-Cell Lung Cancer Cell by Suppressing ERK and STAT3 Signaling Pathways

Figure 1

TF potentiated chemosensitivity to gefitinib in NSCLC cell line PC-9R. (a) TF inhibited human NSCLC cell growth in vitro. Cell survival was determined by MTT assay. (b) PC-9R cells were treated with indicated concentrations of TF for 24 h. Proteins were extracted and subjected to Western blotting assay to evaluate cleavage of caspases and PCNA (left panel); quantitative analysis expression of proteins presented (right panel). Data was presented by for three separate experiments. . (c) Gefitinib inhibited human NSCLC cell growth in vitro. Cell survival was determined by MTT assay. (d) PC-9R cells were treated with vehicle, 8 μM or 17 μM gefitinib and then treated alone or combine with indicated concentration of TF for 24 h. Cell survival was determined by MTT assay. Each point represents the mean of the data of three independent experiments; bars, SD.; .

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