Inhibition of eIF2α Dephosphorylation Protects Hepatocytes from Apoptosis by Alleviating ER Stress in Acute Liver Injury

Figure 5

Inhibition of eIF2α dephosphorylation reduces TG-induced ER stress and apoptosis in LO2 cells. (a) p-PERK, p-eIF2α, ATF4, ATF6, XBP1s, CHOP, and cleaved caspase-3 protein expression at 12, 24, and 48 h post-DMSO (control) or post-TG (0.5 μmol/L) incubation in LO2 cells. (b) Viability of LO2 cells determined by MTS. (c) Bar chart representing the apoptotic index in LO2 cells determined by flow cytometry. (d) p-eIF2α, ATF4, ATF6, XBP1s, CHOP, and cleaved caspase-3 protein expression in control (DMSO pretreatment for 2 h and DMSO incubation for 24 h), salubrinal (salubrinal pretreatment for 2 h and DMSO incubation 24 h), TG (DMSO pretreatment for 2 h and TG incubation 24 h), and salubrinal+TG (salubrinal pretreatment for 2 h and TG incubation 24 h) groups. (e) Viability of LO2 cells determined by MTS. (f) Apoptotic index in LO2 cells determined by flow cytometry. Representative blots from four independent experiments are shown. Histograms represent of four independent experiments. , versus the control group. ^† versus the TG group.

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