Research Article

GDF15 Repression Contributes to 5-Fluorouracil Resistance in Human Colon Cancer by Regulating Epithelial-Mesenchymal Transition and Apoptosis

Figure 2

The low expression of GDF15 in 5-FU-resistant cells was associated with enhanced migration and antiapoptotic ability. (a) Western blotting analysis of MDR1, MRP1, MMP14, N-cadherin, E-cadherin, Bcl-2, cleaved caspase-3, and Bax levels in HCT-15 and HCT-15/FU cells treated with 0.76 mM 5-FU for 48 hours. (b) GDF15 mRNA and protein level in HCT-15 and HCT-15/FU cells treated with 0.76 mM 5-FU for 48 h. Upper: the relative mRNA expression of GDF15 in HCT-15 and HCT-15/FU cells; lower: western blotting analysis of GDF15 and the downstream signaling pathway protein P-Smad2/3 in HCT-15 and HCT-15/FU cells. The data represent the . Statistical significance: .
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