GDF15 Repression Contributes to 5-Fluorouracil Resistance in Human Colon Cancer by Regulating Epithelial-Mesenchymal Transition and Apoptosis

Figure 3

The overexpression of GDF15 resensitized the 5-FU-resistant HCT-15/FU cells to 5-FU. (a) The mRNA expression of GDF15 in HCT-15/FU cells treated with 0.76 mM 5-FU for 0 h or 48 h after being transfected with pCMV-GDF15 or empty vector. (b) Western blotting analysis of GDF15 in HCT-15/FU cells treated with 0.76 mM 5-FU for 0 h or 48 h after being transfected with pCMV-GDF15 or empty vector. (c) The MTS assay to detect inhibition of growth by 5-FU in HCT-15/FU cells treated with 5-FU after being transfected with pCMV-GDF15 or empty vector. (d, e) The cell migration assay of HCT-15/FU cells treated with 0.76 mM 5-FU for 0 h or 48 h after being transfected with pCMV-GDF15 or empty vector. (d) The transwell migration assay (scale bars, 50 μm) and (e) wound healing assay (scale bars, 200 μm). (f) The flow cytometric apoptosis assays of HCT-15/FU cells treated with 0.76 mM 5-FU for 0 h or 48 h after being transfected with pCMV-GDF15 or empty vector. (g) Western blotting analysis of MDR1, MRP1, MMP14, N-cadherin, E-cadherin, Bcl-2, cleaved caspase-3, and Bax levels in HCT-15/FU cells treated with 0.76 mM 5-FU for 48 h after being transfected with pCMV-GDF15 or empty vector. The data represent the . Statistical significance: .

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